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Original Article

AJVS. 2017; 53(2): 6-10

Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa

Ahmed M. El-Ruby, Mamdouh S. Hussein, Wael Bedier, Abdelmonem M. Montaser, Magdy R. Badr, Abdelraouf O. Hegab, Samy M. Zaabel.

In vitro fertilization (IVF) is a necessary procedure in the in vitro production of embryo. Spermatozoa need to undergo capacitation to be able to perform the acrosome reaction which is required for zona pellucida penetration. Different additives are used to aid in that process, such as caffeine and theophylline. In this study, the effects of IVF media supplementation with D- penicillamine, Hypotaurine and epinephrine (PHE) on fertilization and cleavage rates were assessed. Cumulus oocyte complexes (COCs) containing a compact cumulus cells and normal homogenous cytoplasm were selected. In vitro maturation (IVM) was performed in TCM199 supplied with hormones overnight in a culture conditions of 38.5C and 5% CO2 in air. After 20-22 hr. of maturation, oocytes were randomly assigned to five groups of sperm-TALP media (control and four different PHE concentrations) then inseminated. The fertilization rate of the expected embryos were assessed after 22 hr. after staining with aceto-orcin 1% or cultured for 48 hr. to estimate the cleavage rate. The current data revealed that, 25 l/ml PHE significantly (P< 0.05) improved fertilization (55.93 vs 36.96%) and cleavage (46.15 vs 22.22%) rates when compared to control.

Key words: D- Penecillamine, Hypotaurine, Epinephrine, In vitro fertilization, Buffalo

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