The hydrophilic interaction chromatography (HILIC) coupled with UV detection was employed to study retention behaviour and separation of genistein. The genistein is a compound of the isoflavone family found in natural resources in various plants, including herbs, tea, bee products and legumes such as soybeans. A simple, fast and efficient HILIC-UV method was developed for the quantitative and qualitative purposes of genistein in fenugreek seeds, olive leaf, and propolis. The best separation was achieved using a gradient elution of acetonitrile and acetate buffer on ZIC-HILIC column from Merck SeQuant (100 mm×4.6 mm I.D., 5μm) at 35 °C. The genistein was identified with a UV detector at 260 nm. The result demonstrates that the linear range 0.05-5 µgmL-1, high precision (RSD% < 0.5%), and excellent validated values for both LOD (0.0047 µgmL-1) and LOQ (0.0142 µgmL-1).
Key words: Genistein, propolis, fenugreek, flavonoids, olive leaf