Abstract
Objectives: the main objective of the present study is to find an appropriate in-vitro therapy to overcome clinically isolated antibiotic-resistant Salmonella and Shigella species from cases of one-day production layer poultry farm suffering from purulent dysentery and diarrhea. Material and methods: the present study demonstrated chemical synthesis of silver nanoparticles (AgNPs) via chemical reduction method and investigation for their antibacterial effect against the isolated bacteria by determining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Results and Conclusions: of 32 examined samples, 8 (25%) antibiotic-resistant isolates were isolated and identified including: S. flexeneri, S. typhimurum, S.poona, S. bodyi, S. typhimurium, S. montevideo, S. sonnei, S. enteritidis. Spherical AgNPs of 10-25nm in size were synthesized and The AgNPs at a concentration of 16 μg ml-1 were found to have both bacteriostatic and bactericidal effects in the case of S. montevideo (layer chicken egg), S. sonnei (layer chicken feces), S. enteritidis (layer duck egg) however the AgNPs at a concentration of 8 μg ml-1 were found to have both bacteriostatic and bactericidal effects in the case of S.poona (layer chicken feces), S. bodyi (layer chicken feces), S. typhimurium (layer chicken feces).
Key words: AgNPs, MIC, MBC, Salmonella, Shigella, antibacterial, poultry.
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