Spectrum of Microbial Flora in Diabetic Foot Ulcer and Its Antibiotic Sensitivity Pattern in Tertiary Care Hospital in Ahmedabad, Gujarat
Manisha Jain, Mitesh H Patel, Nidhi K Sood, Dhara J Modi, M M Vegad.
Introduction: A Prospective study “Spectrum of Microbial flora in diabetic foot ulcer and its antibiotic sensitivity pattern” was carried out in a tertiary care hospital, Ahmedabad on 125 patients in which 85 were male patients and 40 were female patients.
Material and Methods: Swabs samples were collected from the edge and margins of ulcers and organism were identified by gram staining culture and biochemical reactions.
Results: Out of 125 specimens 108 specimens showed growth of organisms. Total 157 aerobic organisms were isolated from culture positive specimens. It represents an average of 1.25 organisms per case. Among these organisms, 130 gram negative and 27 gram positive organisms were isolated. Pseudomonas aeruginosa (30.57%) was predominant organism followed by Klebsiella spp. (22.29%). Staphylococcus aureus were 12.74% in which Methicillin resistant S. aureus (MRSA) was 55%.
Conclusion: incidence of growth was 86.4% in which Pseudomonas aeruginosa (30.57%) is most common isolate. Organisms in mixed infections showed multidrug resistance as compared to single isolated strain. Diabetic foot infections are polymicrobial in nature. As the Wagner’s grade increased, the prevalence of isolates also increased.
Key words: Diabetic Foot Ulcer, Polymicrobial Ulcer, Wagner’s grade