Essential oil of Litsea petiolata Hook. f. was extracted from their leaves collecting from Chiang Rai province, Northern Thailand. The extraction was performed for 4 h by using hydrodistillation with Clevenger system. The essential oil composition was further analyzed by gas chromatography-mass spectrometry. As a result, a total of 74 volatile components were identified. Representing 96.94% of the total oil with 2-ketones is the major compound group. The key identified volatile components were 2-undecanone (79.52%), 12-tridecen-2-one (6.26%) and 2-nonanone (2.46%), respectively. The essential oil of L. petiolata Hook. f. leaves showed good antibacterial activity against tested Gram-positive and Gram-negative human pathogenic bacteria including Bacillus cereus DMST 5040, B. subtilis TISTR 008, Staphylococcus aureus DMST 8840, S. epidermidis DMST 15505, S. agalactiae DMST 17129, Escherichia coli DMST 4212, Salmonella typhimurium DMST 562, Vibrio cholerae DMST 2873 and V. parahaemolyticus DMST 21243. The zone of inhibition was obtained from 9.54±1.24 to 18.07±2.19 mm. The minimum inhibitory concentration (MIC) values were within the antibacterial activity range and varied between 31.25 and 500 μg/mL. The essential oil of L. petiolata Hook. f. leaves showed maximum antibacterial activity against S. aureus DMST 8840 and E. coli DMST 4212. This study indicates that L. petiolata Hook. f. leaf oil could be employed as a natural medicinal application in antibacterial treatments.
Key words: Litsea petiolata Hook. f., essential oil, antibacterial, composition, GC-MS
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