Jatropha multifida is a plant whose sap is used in traditional Beninese medicine as haemostatic. Previous work has shown are efficiencies in rapidly causing blood clotting and stopping bleeding in normal rats. The aim of this study was to investigate the haemostatic effects of J. multifida sap (SJM) on rats with coagulation disorders.
In vivo haemostatic tests were performed on 14 Wistar albino rats including 7 normal rats and 7 warfarin-treated rats (2mg / kg orally for 4 days). After ketamine anesthesia (80 mg / kg) intramuscularly and local application of 2% xylocaine (10 mg / kg); Superficial cuts, saphenous vein and femoral vein were performed simultaneously on the two hind legs of each rat. SJM was applied locally on one of the legs and placebo (0.9% NaCl) on the other. Bleeding Times (TS) were recorded in each case.
In normal rats, the results obtained indicated that the SJM topical application significantly reduced TS regardless of the type of cut. Reduction percentages were 39.06%, 46.68% and 47.89%, respectively, with superficial cuts, saphenous vein and femoral vein. In rats pretreated with warfarin, the TS reduction percentages were 71.52%, 61.54% and 66.44%, respectively, with superficial cuts, saphenous vein and femoral vein. The application of SJM significantly decreased TS compared to placebo.
SJM has been shown to be effective in stopping hemorrhage in normal rats and those with coagulation disorders.
Key words: warfarin, hemostatic, rats, J. multifida.
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