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Review Article

Loop-Mediated Isothermal Amplification Assay (LAMP): A Rapid Tool for Diagnosis of Foodborne and Zoonotic Pathogens: A Review

Ravindra J. Zende, Deepak P. Kshirsagar, Vilas M. Vaidya, Rupesh N. Waghamare, Riddina P. Todankar,Aparna H. Shirke.

Loop-mediated isothermal amplification (LAMP) assay was introduced in the year 2000 by Notomi, as a highly sensitive, specific and cost-effective technique for microbial identification. In contrast to the polymerase chain reaction (PCR) technology in which the reaction is carried out with a series of alternating temperature steps or cycles, isothermal amplification is carried out at a constant temperature and does not require a thermal cycler. LAMP, a simple DNA amplification technique, with its field amenable nature has been used to detect a variety of pathogens including viruses, fungi, bacteria and parasites and in most of the cases it surpasses polymerase chain reaction. This review emphasis on importance of LAMP for successful detection of pathogens from minimally processed samples or in presence of clinical sample matrices. This feature of LAMP may be useful in low resource or field settings where conventional DNA or RNA extraction prior to diagnostic testing may be impractical.

Key words: LAMP, PCR, Foodborne and Zoonotic Pathogens

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American Journal of Diagnostic Imaging


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