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Influence of Different Level of Egg Yolk on Cryopreservation of Hariana Bull Spermatozoa

Vikas Sachan, Vipin Sonker, Atul Saxena.


This study has been conducted to evaluate the effect of inclusion of different levels of egg yolk (12%, 16% and 20%) in Tris-diluent on the viability, progressive motility, membrane integrity and acrosomal damage of Hariana bull spermatozoa at various stages of cryopreservation. 32 seminal ejaculates from 4 bulls (8x4) were subjected to cryopreservation and thawing process and evaluated with various tests at three different stages of freezing and thawing process (After dilution, after equilibration and after thawing). Statistical analysis was done as per the standard procedure (Snadocor and Cocharan, 1967). The mean percentage of live spermatozoa, progressive motile, HOST positive and sperms with intact acrosome were significantly higher (P

Key words: Hariana Bull, Egg Yolk, Cryopreservation, Viability, Progressive Motility, Membrane Integrity, Acrosomal Integrity

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