In the present study the methanol extracts of Tinospora sinensis Lour (Merr.) was studied for alpha (α)-amylase and alpha (α)-glucosidase inhibition using an in vitro model. The plant extracts was also examined for its antioxidant activity by using free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging method, ABTS radical scavenging ability, reducing power capacity, estimation of total phenolic content, flavonoid content and flavonol content.
The study revealed that the different concentrations of the extract possessed a very good amount of total phenolics, flavonoid and flavonol and exhibit potent radical scavenging activity using DPPH and ABTS as substrate. The methanol extract exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value 0.75µg and 0.80 µg dry extract respectively and well compared with standard acarbose drug. Thus, it could be concluded that due the presence of antioxidant components the plant extract have well prospective for the management of hyperglycemia, diabetes and the related condition of oxidative stress. This knowledge will be useful in finding more potent antidiabetic principle from the natural resources for the clinical development of antidiabetic therapeutics.
Key words: Tinospora sinensis , Methanol extract , Antioxidant activities, α-amylase inhibitory activity , α-glucosidase inhibitory activity