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Headspace-gas chromatography/mass spectrometry analysis of methanol in blood

Dilek Salkim Islek, Sibel Ramadanoglu.

Abstract
A headspace gas chromatographic-mass spectrometric (HS-GC/MS) method was developed for detection of ethanol and methanol in blood and quality control and validation was performed. The linearity ranges of the method were 0-2g/L for ethanol and methanol. The detection of limit was 0.03g/L for ethanol and methanol. The quantitation of limit was 0.05g/L for ethanol and methanol. The range of recoveries was between 94%-120% for both ethanol and methanol.
In our study case; A 60-year-old woman was found unconscious at home, with a needle mark on her leg. A bottle of purple liquid was found next to the body. She was presented involving a transient loss of consciousness resulting from self-administered denaturated alcohol (methylated alcohol). Doctor demanded us to determine methanol and ethanol in blood and purple liquid . The compounds were identified and quantified by headspace gas chromatography coupled to mass spectrometry

Key words: Methanol, ethanol, HS-GC/MS, blood


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