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Original Article

Inducible Antimicrobial Compounds (Halal) Production in Honey Bee Larvae (Apis mellifera) from Rumaida, Taif by injecting of various dead Microorganisms extracts

Abd-ElAziem Farouk, N Thoufeek Ahamed, Othman AlZahrani, Akram Alghamdi, AbdulAziz Bahobail.

Cited by 5 Articles

In the current study, the antimicrobial compounds in induced Honey bee larvae (Apis mellifera) as Halal product from the Taif region were extracted from hemolymph and their minimum inhibitory concentrations (MIC) were evaluated. Five different microbes isolated from pure cultures, namely Escherichia coli, Bacillus subtilis, Pseudomonas aureus, Candida albicans and Micrococous were used in this study by the agar diffusion assay method. The antibacterial activity of induced honey bee larvae was studied against the 5 above mentioned organisms and the MIC was denoted from the inhibition. The inducible antimicrobial protein compounds extracted from honey bee larvae hemolymph, smashing out, after one day at room temperature were used for minimum inhibitory concentration. Studies showed appreciable antimicrobial activity (6±1) when the larvae were injected with 15µl Micrococus leutus total protein extract and when injected with the same concentration (i.e. 15µl Pseudomonas aureus), they showed a weaker activity (4±1) against Candida albicans. The Amoxycillin was used as a standard positive control. The negative control of the Phosphate buffer solvent (PBS) and larvae did not show any activity against all five organisms. All dilutions were executed, minimum inhibitory concentration studies ranging from 10-1 to 10 -4 did not show any activity. The most inhibition was obtained with induced young larvae at various ages (L4 and L5) against Candida albicans.

Key words: Antibacterial activity, Apis mellifera, honey bee hemolymph, minimum inhibitory concentration, MIC,

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