Abstract

Background:
Pasteurella multocida is a Gram-negative bacterium responsible for various diseases in animals, including Haemorrhagic Septicaemia (HS). Oxford Nanopore Technologies’ platform streams sequencing data immediately, allowing for on-the-fly analysis. This capability enables researchers to monitor experiments in real-time and make prompt decisions, which is particularly beneficial in clinical diagnostics and outbreak surveillance.

Aim:
This study presents the genome assembly of two P. multocida isolates using long-read sequencing technology provided by Oxford Nanopore Technologies.

Methods:
This method involves passing DNA strands through nanopores and detecting changes in electrical current to determine the nucleotide sequence. The long-read capability of ONT's technology allows for sequencing of extensive DNA fragments, which is particularly beneficial for resolving complex genomic regions and achieving high-contiguity genome assemblies.

Results:
The study achieved high-quality, single-contig genomes with sizes of 2.35 Mbp and 2.29 Mbp, and completeness scores exceeding 99% (BUSCO). These assemblies provide valuable resources for functional analyses, enhancing the understanding of virulence factors and the pathogenesis of Haemorrhagic Septicaemia in Indonesian livestock.

Conclusion:
The study successfully assembled the genomes of two P. multocida isolates from Lampung (2952) and Kupang, Nusa Tenggara Timur (NTT), Indonesia, using Oxford Nanopore Technologies' long-read sequencing. The resulting single-contig genomes, measuring 2.35 Mbp and 2.29 Mbp respectively, exhibited high completeness scores (BUSCO: >99%). These high-quality assemblies enhance genomic contiguity, providing valuable resources for functional analyses aimed at understanding the virulence factors and pathogenesis of Haemorrhagic Septicaemia in Indonesian livestock.

Key words: Genome assembly, Hemorrhagic septicemia, Oxford Nanopore Technologies, P. multocida, Whole genome sequencing