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Phytochemical screening and study of anti-oxidant, anti-microbial, anti-diabetic, anti-inflammatory and analgesic activities of extracts from stem wood of Pterocarpus marsupium Roxburgh

Dipak Raj Pant, Narayan Dutt Pant, Dil Bahadur Saru, Uday Narayan Yadav, Dharma Prasad Khanal.


Abstract

Aims: The main aims of the study were to screen the phytochemical constituents and to study the anti-oxidant, anti-microbial, anti-diabetic, anti-inflammatory and analgesic activities of extracts from stem wood of Pterocarpus marsupium.
Methods: Ethanol and acetone & isopropyl alcohol (IPA) (1:1) extracts of stem wood of Pterocarpus marsupium were subjected to phytochemical screening and analysis of biological activities in 2016. Phytochemical screening included the qualitative test by color reactions and quantitative estimation of total polyphenolic and flavonoid contents. Anti-oxidant assay was carried out by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method, anti-microbial activity testing by cup diffusion method, anti-diabetic test evaluation by oral glucose tolerance test in mice, anti-inflammatory effect evaluation by hind paw edema method in mice and analgesic test evaluation by chemical writhing method in mice.
Results: The results of study revealed that P. marsupium is a source of various phytoconstituents such as alkaloids, glycosides, saponins, tannins, proteins, carbohydrates, cardiac glycosides, flavonoids and terpinoids. Both the acetone & IPA extract and ethanol extract of stem wood of Pterocarpus marsupium, exhibited the dose dependent antioxidant activity. Acetone & IPA extract showed antibacterial activity against gram positive bacteria, while the ethanolic extract was found to possess anti-diabetic activity. The anti-diabetic activity of the extract was found to be time and dose dependent. Similarly, the acetone & IPA extract was found to have anti-inflammatory activity, which was also time and dose dependent. Further, the ethanolic extract showed analgesic activity, which was dose dependent. The ethanolic extract was found to be non-toxic.
Conclusions: Thus this study laid sufficient background for the further research on extracts from stem wood of Pterocarpus marsupium for identification, subsequent purification and isolation of compounds having anti-bacterial, anti-diabetic, anti-inflammatory and analgesic activities.

Key words: Pterocarpus marsupium, phytochemicals, anti-bacterial, anti-diabetic, anti-inflammatory, analgesic






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