High yields of extracted DNA from animal tissues for number of molecular research work is depend on acquiring tissues that should not highly degraded and must give a sufficient yield of DNA. Current experiments were performed on various tissues preservations, so, later on the DNA extraction can be done to get high quality and quantity of DNA. 90% alcohol, 8% formalin and -400 C deep freezing were used for 5 days, 20 months and 28 months each to stay fish tissues. DNA was extracted from preserved tissues after specified intervals, quantification was performed using Nano-Drop Spectrophotometer and PCR amplifications via random primers. Genomic DNA extraction method was used for high yield, quality and suitability for RAPD markers amplification in Sperata seenghala. Phenol-chloroform-isoamyl method agreed clear and sharp DNA quality preserved in 90% alcohol and the purity near 1.8. However, formalin preserved samples were gave low quantity of DNA as 8.0 ng/µl with 1.35 ratios. This method proved successful results tissues preserved in 90% alcohol and -40 0C preservatives, whereas, other extraction protocol i.e., formalin failed.
Key words: Genomic DNA extraction, chloroform-isoamyl-alcohol, molecular markers, tissues preservatives, DNA integrity.
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