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Original Article



Comparative analyses of genomic DNA extracted from freshwater fish tissues preserved in formaldehyde and alcohol in different periods of time

R. K. Garg, Khushboo Sengar, R. K. Singh.




Abstract
Cited by 2 Articles

High yields of extracted DNA from animal tissues for number of molecular research work is depend on acquiring tissues that should not highly degraded and must give a sufficient yield of DNA. Current experiments were performed on various tissues preservations, so, later on the DNA extraction can be done to get high quality and quantity of DNA. 90% alcohol, 8% formalin and -400 C deep freezing were used for 5 days, 20 months and 28 months each to stay fish tissues. DNA was extracted from preserved tissues after specified intervals, quantification was performed using Nano-Drop Spectrophotometer and PCR amplifications via random primers. Genomic DNA extraction method was used for high yield, quality and suitability for RAPD markers amplification in Sperata seenghala. Phenol-chloroform-isoamyl method agreed clear and sharp DNA quality preserved in 90% alcohol and the purity near 1.8. However, formalin preserved samples were gave low quantity of DNA as 8.0 ng/µl with 1.35 ratios. This method proved successful results tissues preserved in 90% alcohol and -40 0C preservatives, whereas, other extraction protocol i.e., formalin failed.

Key words: Genomic DNA extraction, chloroform-isoamyl-alcohol, molecular markers, tissues preservatives, DNA integrity.






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