Abstract

In the recent years, tissue culture has emerged as a promising technique to obtain genetically pure elite populations under in vitro conditions. The Cleome viscosa are used in traditional systems of medicine for the treatment of many diseases in human. The present study aims to investigate the role of assorted plant growth regulators (PGRs) on in vitro propagation and comparison of similar and dissimilar compounds of wild plant C. viscosa. Nodal explants of 1.5-2.0 cm were used to induce multiple shoots in Murashige and Skoog (MS) medium supplemented with various concentration of different plant growth regulators (PGRs) such as 6-Benzylaminopurine (BAP), Naphthalene-3-acetic acid (NAA), Indole-3- acetic acid (IAA) and the bioactive constituent of wild and in vitro propagated C. viscosa plant was compared by analyzing polar and non polar extract of both the plants using Gas Chromatography - Mass Spectrometry (GC-MS) analysis. Multiple shoots were initiated within 28 days of inoculums and the various concentration of PGR had a significant role in the number of shoot formed and the in vitro regeneration of explants. The regenerated plantlets showed no morphological differences from the wild plant but the GC-MS analysis of ethanol extract showed the presence of eight compounds in wild plant and six in micropropagated while chloroform extract showed ten compounds in both plants.

Key words: In vitro propagation, MS medium, plant growth regulators, GC-MS analysis