Research to identify the possible potential of ethyl acetate extract of symbiotic fungi from marine sponge Neopetrosia chaliniformis AR-01 as producer of cytotoxic and antibacterial compounds has been conducted. Symbiotic fungi of N. chaliniformis were isolated by casting method using Sabouraud Dextrose Agar (SDA) and purified by the scratch method. Pure isolated fungi then was cultivated using rice as media at temperature of 25-27 ° C for 4-8 weeks and extracted using ethyl acetate solvent. The ethyl acetate extracts were tested as cytotoxic using Brine Shrimp Lethality Test (BSLT) and tested as an antibacterial against pathogenic bacteria of Gram positive (Bacillus subtilis and Staphylococcus epidermidis) and Gram negative (Escherichia coli and Salmonella typhosa) using agar diffusion method. There are 13 symbiotic fungi of marine sponge N. chaliniformis that had been isolated. The screening result of the cytotoxic activity showed that 76.92 % or 10 fungi isolates were classified as cytotoxic with LC50 10 mm. Based on the screening results, it can be concluded that ethyl acetate extracts of the symbiotic fungi of marine sponge N. chaliniformis are a potential source for producing anticancer and antibacterial compounds.
Key words: Marine sponge, symbiotic fungi, Neopetrosia chaliniformis, cytotoxic activity, antibacterial activity.