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Original Article

JVS. 2017; 1(1): 100-113


Elimination of Alfalfa mosaic virus (AMV) from infected potato leaves (Solanum tuberosum) cv. Ditta by embryonic calli

Maha Ahmed Maher El-Abhar, Moustafa Ahmed Salama El-Kady, Khaled Mohamed Ghanem, Hussieny Abd-Elhak Bosila.

Abstract
Elimination of alfalfa mosaic virus (AMV) from infected potato (Solanum tuberosum L.) CV. Ditta via embryonic calli was evaluated. AMV was isolated from potato plants and characterized. Virus-infected plants, grown under greenhouse conditions, were used as a source for virus elimination. Leaves excised from green parts of infected potato produce pro-embryogenic masses (PEMs) were controlled by MS basal medium supplemented with specific growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) at concentration of 1,2,3,4,5 mgl-1 and combination of 5mgl-1 2,4-D + 0.5 of Kin, benzylaminopurine (BAP), TDZ and Zeiten mgl-1. After incubation period of 30 35 days, 100 % of creamy-green granular callus with 81.17% pro-embryonic mass were obtaind with 5 mgl-1 2,4-D + 0.5 mg-l Kinetin (Kin). Vice versa, the combination of 5 mgl-1 2,4-D +0.5 mgl-1Thidiazuron (TDZ) had the lowest effect (11.11 %) of brown rigid callus formed. Pro-embryo development stages were subjected to histological study via light microscopy and serological tests by Enzyme-linked immunosorbent assay (ELISA) technique. Outcome of this study demonstrated the effectiveness of embryonic calli at various stages of the pro-embryo development could be used as a procedure for eliminate of AMV from infected leaves as a source material.

Key words: Key words: Potato (Solanum tuberosum), embryonic callus, growth regulators, alfalfa mosaic virus (AMV)



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