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Original Article

JVS. 2017; 1(1): 100-113

Elimination of Alfalfa mosaic virus (AMV) from infected potato leaves (Solanum tuberosum) cv. Ditta by embryonic calli

Maha Ahmed Maher El-Abhar, Moustafa Ahmed Salama El-Kady, Khaled Mohamed Ghanem, Hussieny Abd-Elhak Bosila.

Elimination of alfalfa mosaic virus (AMV) from infected potato (Solanum tuberosum L.) CV. Ditta via embryonic calli was evaluated. AMV was isolated from potato plants and characterized. Virus-infected plants, grown under greenhouse conditions, were used as a source for virus elimination. Leaves excised from green parts of infected potato produce pro-embryogenic masses (PEMs) were controlled by MS basal medium supplemented with specific growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) at concentration of 1,2,3,4,5 mgl-1 and combination of 5mgl-1 2,4-D + 0.5 of Kin, benzylaminopurine (BAP), TDZ and Zeiten mgl-1. After incubation period of 30 35 days, 100 % of creamy-green granular callus with 81.17% pro-embryonic mass were obtaind with 5 mgl-1 2,4-D + 0.5 mg-l Kinetin (Kin). Vice versa, the combination of 5 mgl-1 2,4-D +0.5 mgl-1Thidiazuron (TDZ) had the lowest effect (11.11 %) of brown rigid callus formed. Pro-embryo development stages were subjected to histological study via light microscopy and serological tests by Enzyme-linked immunosorbent assay (ELISA) technique. Outcome of this study demonstrated the effectiveness of embryonic calli at various stages of the pro-embryo development could be used as a procedure for eliminate of AMV from infected leaves as a source material.

Key words: Key words: Potato (Solanum tuberosum), embryonic callus, growth regulators, alfalfa mosaic virus (AMV)

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