Objective: To identify the effect of tcotrienols on adventitial changes induced by high cholesterol diet in
descending thoracic aorta of rabbits.
Study Design: Laboratory based randomized control trial.
Place and Duration of Study: The Anatomy department of Army Medical College Rawalpindi in collaboration
th th with National Institute of Health, Islamabad. The study commenced on 10 March 2009 and completed on 10
Materials and Methods: Thirty adult male New Zealand White rabbits were randomly divided into three equal
groups (I, II & III). Group-I consumed standard NIH diet while group-II was fed 2% high cholesterol diet. Group-III
animals were given the same diet as to group II, however, tocotrienols 6 mg/kg body wt/day were also added to
the diet. Following six weeks of experiment, aorta of every animal was dissected. Cross sections were taken
from descending thoracic aorta and processed for light microscopic examination. In H&E and verhoeff stained
slides, adventitial histomorphological changes were compared among the three groups.
Results: In rabbits on standard diet, tunica adventitia was a thin layer composed of loose network of collagen
and elastic fibers which lacked lamellar pattern as that of media. Adventitial cells were relatively scanty. In
contrast, aortic adventitia in group-II was thickened with increased number of inflammatory cells characterized
by central round nucleus and foamy cytoplasm. Above mentioned histological changes were present in group-
III but were of lesser degree than group-II. MeanąSD thickness of adventitia and inflammatory cells score was
significantly greater in group-II &III when either was compared with group-I. However, group-III showed 19%
Adventitia, High Cholesterol Diet, Rabbit Aorta, Tocotrienols