Abstract

Objective: To explore the anti-cancer potential of Capsaicin on Henrietta Lacks HeLa Human cervical cancer cell line.
Methodology: This in-vitro study was carried out from 12th December 2023 to 12th February 2024, translating lab results into IC50 (half-maximal inhibitory concentration) values depended on MTT assay outcomes for evaluating capsaicin concentration effects on HeLa cell lines while performing gene expression studies through RT-qPCR analysis of apoptotic mediator genes (Intrinsic Biomarkers Caspase)
Results: Using IC50 revealed capsaicin at 12.1 uM effectively induced pro-apoptotic biomarkers Caspase-3 and Caspase-9 in HeLa cells at relative gene expression levels of 11.2 and 12.9 respectively.
Conclusion: The data confirmed robust pro-apoptotic anti-cancer properties of capsaicin while indicating diagnostic uniqueness against HeLa cell lines. Future investigations need to discover the main pathways through which capsaicin fights cancer in HeLa cell lines.

Key words: HeLa, capsaicin, pro-apoptotic potential, anti-cancer activity, natural chemotherapeutics.