Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Article

J App Pharm Sci. 2016; 6(12): 020-027


Enhancement of novel extracellular bacteriocin production by media optimization using LAB isolate from meat

Enas Nabil Danial, Salha Hassan Mastour Al-Zahrani, Zahra Al-Hassan Mohammad Al-Mahmoudi.

Abstract
Bacteriocins of lactic acid bacteria have been widely studied in recent years; however, there are relatively few studies that describe their chemical structure. This may be due to the many challenges associated with the purification of these antimicrobial peptides. This study was amid to isolate lactic Acid Bacteria (LAB) from meat in Jeddah - Saudi Arabia on (MRS) medium. Forty isolates of LAB showed antibacterial activities against indicator bacteria Gram positive bacteria S.aureus ATCC25923, and Gram negative bacteria E.coli ATCC25422, K.pneumonia ATCC700603, P.aeruginosa ATCC27583. Also antibacterial activities was screened by agar well diffusion method, results showed that ten out of 40 (25%) of the tested isolates have antibacterial activities against all indicator bacteria. The isolates of LAB with the highest antimicrobial effect were identified on the basis of its genetically sequencing. Results revealed that the M8 isolate have 96% identity with Leuconostoc mesentroides and was the highest bacteriocin producer. So it has been selected for the current investigation. The effect of different factors such as (inoculums concentrations, incubation temperature, incubation period, pH and aeration) on the production of bacteriocin was studied. The best condition for the production of highest amount of bacteriocin was incubation of static culture at 35C for 24 hr and pH 6.2.

Key words: bacteriocin , Lactic Acid Bacteria, isolation, production



Similar Articles

Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny.
Azari H
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 111-123

Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord.
Bauchet L, Poulen G, Lonjon N, Vachiery-Lahaye F, Bourinet E, Perrin FE, Hugnot JP
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 103-110

Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded Under Neurosphere Culture.
Koopaei NN, Schmittgen TD, Reynolds BA, Azari H
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 87-94

Post-synthesis modification of covalent organic frameworks for ultrahigh enrichment of low-abundance glycopeptides from human saliva and serum.
Xie Z, Yan Y, Tang K, Ding CF
Talanta. 2022; 236(): 122831

Isolation and Culture of Adult Hippocampal Precursor Cells as Free-Floating Neurospheres.
Rust R, Walker TL
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 33-44


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites