Abstract

Background: Gaok chicken spermatozoa that is diluted and stored at freezing temperatures will reduce spermatozoa motility. The specific target to be achieved by this research is increasing the population by improving the quality of frozen semen with the addition of green tea extract in the egg yolk milk diluent. EGCG contained in green tea extract acts as an antioxidant in the cooling and freezing process of chicken semen.
Aim: This study examines the addition of green tea extract in fructose egg yolk milk diluent to improve chicken spermatozoa quality before and after freezing, with the goal of enhancing the efficacy of artificial insemination programs to increase the Gaok chicken population.
Methods: This study consisted of 4 groups, T0 the control group: fructose egg yolk milk diluent without green tea extract + semen, TI: fructose egg yolk milk diluent + green tea extract dose 0.05 mg/100 ml diluent + semen, T2: fructose egg yolk milk diluent + green tea extract dose 0.10 mg/100 ml diluent + semen and T3: fructose egg yolk milk diluent + extract green tea dose 0.15 mg/100 ml diluent + semen.
Results: The analysis of variance (P < 0.05) showed no significant effects at T0, T1, and T3, indicating that T2, the treatment group supplemented with extract green tea (Camellia sinensis), produced the most notable results. This group maintained is better than another group on parameters sperm motility, viability, plasma membrane integrity, and minimized necrosis, alongside increased catalase enzyme and SOD activity.
Conclusion: The addition of green tea leaf extract subsequently reduced MDA levels. The addition of green tea extract dose 0.10 mg/100 ml of egg yolk milk diluent can as an antioxidant in maintaining the quality of post-thawing Gaok chicken’s spermatozoa.

Key words: Antioxidant levels, conservation, freezing, gaok chicken, semen quality