Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Article



Comparative proteome analysis and thermal stress induced changes in the embryo of poly- and bi-voltine strains of Bombyx mori

Punyavathi Punyavathi, Muzafer A Bhat, Manjunatha H Boregowda.

Abstract
Proteins are the ultimate operating molecules, producing the physiological effect in all the stages of an organism. As a consequence, proteome of the embryo play a pivotal role in determining the biological structures besides cellular organization and morphogenetic movements that occur during embryo development. But, changes in the environment temperature for a few hours whether that alter the embryonic development, protein expression and hatching of infants are unknown in Bombyx mori L. Hence, eggs of poly- and bi-voltine breeds were exposed to heat shock temperature of 35, 40 and 45C for 2 h to measure tolerance level and differential expression of proteins in different age of the embryos. To facilitate comparative analysis among different breeds a comprehensive proteome map was constructed considering the protein profile of different days embryos. Interestingly, ~10 to 21 protein bands were recorded during embryo development - from ovipostion till hatching - along with few new protein bands on day-6, which were differing in their intensity in different silkworm strains. Interestingly, no change in the protein pattern was observed in different days embryos exposed to thermal stress at 35 and 40C. But degradation of 18 and 19 kDa proteins were recorded in all the days of embryos exposed to 45C while four major protein bands remain unaltered. Further, the percent of hatching was slightly declined in 35 and 40C and zero hatching was recorded in all the stages of eggs HS at 45C. This clearly indicates that differential expression/degradation of proteins as a response to thermal stress not only influences the growth and development but also determine hatching of embryo. Accordingly, we suggest that embryonic protein map constructed in the present study shall be a valid reference for comparative analysis and the hatching of embryos as one of the key traits to measure the rate of thermotolerance in the existing or new strains/breeds of B. mori.

Key words: Bombyx mori, Densitometry, Embryo, Global warming, Heat shock, Proteome.



Similar Articles

Does the Covid-19 pandemic affect faith-based investments? Evidence from global sectoral indices.
Dharani M, Hassan MK, Rabbani MR, Huq T
Research in international business and finance. 2022; 59(): 101537

Chemometric analysis of the global pattern of volatile organic compounds in the exhaled breath of patients with COVID-19, post-COVID and healthy subjects. Proof of concept for post-COVID assessment.
Zamora-Mendoza BN, Díaz de León-Martínez L, Rodríguez-Aguilar M, Mizaikoff B, Flores-Ramírez R
Talanta. 2022; 236(): 122832

Geographic distribution and time trend of human exposure of Di(2-ethylhexyl) phthalate among different age groups based on global biomonitoring data.
Qu J, Xia W, Qian X, Wu Y, Li J, Wen S, Xu S
Chemosphere. 2022; 287(Pt 2): 132115

Adverse consequences of emotional support seeking through social network sites in coping with stress from a global pandemic.
Islam AKMN, Mäntymäki M, Laato S, Turel O
International journal of information management. 2022; 62(): 102431

Diffusion tensor imaging of the hippocampus reflects the severity of hippocampal injury induced by global cerebral ischemia/reperfusion injury.
Wang WZ, Liu X, Yang ZY, Wang YZ, Lu HT
Neural regeneration research. 2022; 17(4): 838-844


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites