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Isolation of Spermatogonial Stem Cell and Its Viability in Mice by Different Enzymatic Digestion Methods

R. Kaavya, T. A. Kannan, Sabiha Hayath Basha, S. Vairamuthu, Geetha Ramesh, B. Justin William.




Abstract

The mouse SSCs can be isolated using two step enzymatic digestion method using different enzyme combinations. To conclude, Type II enzymatic digestion method yielded cells, more in number and viable percentage in both pre-pubertal and post-pubertal age groups, than in Type I enzymatic digestion method. The present study provided basic protocol for isolation of maximum yield of functionally viable, spermatogonial stem cell population for further research or therapy.

Key words: Spermatogonial Stem Cells, Yield, Viability, Enzymatic Digestion, Mice






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