Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Article



Partial purification and characterization of alkaline protease enzyme from Pseudomonas aeruginosa for tannery in Bangladesh

Mohammad Showkat Mahmud, Md. Gahangir Alam, Shamsun Nahar, Md. Jakir Hossain.

Abstract
An extracellular protease producing bacteria was isolated from poultry waste deposited site at Dhamrai, Dhaka. The isolated bacteria was confirmed as Pseudomonas aeruginosa after performing different morphological and biochemical tests The extracellular protease from the isolated P. aeruginosa was partially purified and characterized. The enzyme was purified by ammonium sulphate precipitation and DEAE cellulose column chromatography. SDS-PAGE was performed and the presence of protease enzyme was confirmed and the molecular weight of protease was determined as 66 KDa. Dehairing treatment with the isolated protease enzyme showed higher activity comparing with some available protease of other bacterial sources as well as conventional chemical method. Effect of pH, temperature, salt-ions and other effector molecules on protease from isolated P. aeruginosa was also examined. The enzyme showed higher activity at pH 8.0 and optimum temperature was found as 35-45˚C. The activity of the enzyme slightly decreased in presence of salt-ions (Mg++, Ca++, Zn++, Mn++, Na+, K+) and inhibitors (EDTA, 2-mercaptoethenol, DMSO, SDS, Ethanol, Methanol). The deactivating effect of 2-marceptoethanol indicated the enzyme as cystine protease.

Key words: Pesudomonas aeruginosa; protease enzyme; SDS-PAGE; tannery



Share this Article


Advertisement
Journal of Behavioral Health

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.