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J App Pharm Sci. 2017; 7(4): 209-212


Antibacterial Activity of Endophytic Fungi Isolated from Mangrove Plant Sonneratia griffithii Kurz

Dian Handayani, Harrizul Rivai, Monica Hutabarat, Roslaili Rasyid.




Abstract

ABSTRACT
The aim of this study was focused on the antibacterial activity of endophytic fungi from leaf, bark and root of mangrove Sonneratia grifithii Kurz, collected from Bungus, West Sumatra.
Isolation of endophytic fungi was based on direct planting method with sabouraud dextrose agar (SDA) as growth medium. Twelve isolates fungi strains were obtained this mangrove. They were cultivated on sabouraud dextrose broth (SDB) for 4 weeks and they are fractioned using ethyl acetate and buthanol. Ethyl acetate and butanol extracts was analyzed for antibacterial activity. The fungi strains which showed the maximum activity were identified by macroscopic and microscopic examination. Twelve (50%) extracts of endophyte fungi strains were able to inhibit the growth of pathogenic bacteria such as, Staphylococcus aureus and Escherichia coli. Endophytic fungi strains that have higher antibacterial activity derived from the root than from leave and bark Sonneratia griffithii Kurz. This work concluded that endophytic fungi strains of Sonneratia griffithii Kurz can be developed as a new source of antibiotic compounds.

Key words: Endophytic fungi, mangrove, Sonneratia griffithii Kurz, antibacterial activity






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