Screening of anticancer and antibacterial activities of ethyl acetate extract of symbiotic fungi from marine sponge Acanthostrongylophora ingens was conducted. Sponge A.ingens was collected from Mandeh island, West Sumatra. Isolation of symbiotic fungi was done by pour plate method using sabouraund dextrose agar (SDA) medium and purified by scratch method. Pure isolates were cultivated in rice medium for 6 week at room temperature. Result of cultivation was extracted with ethyl acetate. Each extract further was screened for cytotoxic activity with BSLT method using shrimp larva of Artemia salina Leach and antibacterial activity against Basillus subtilis, Staphylococcus epidemidis, Salmonella typosa and Escherichia coli using agar diffusion method. The ethyl acetate extract of fungi strains which showed interesting results on the bioactivity screening was performed a phytochemical test to determine the chemical content.
There were 8 isolated symbiotic fungi strains from the sponge. Based on the screening cytotoxic result, LC50 value from each extract were IB121 isolate (44.59 ppm), IB131 isolate (11.52 ppm), IB142 isolate (662.2 ppm), IB161 isolate (221.82 ppm), IB102 isolate (172.66 ppm), IB141 isolate (58.56 ppm), IB101 isolate (3.801 ppm) and IB151 isolate (0.53 ppm). Based on the result of antibacterial activity screening against B. substilis, S. epidemidis, S. typosa and E. coli, the biggest inhibition growth was resulted by fungi strain of IB141 with diameter inhibition of 14.4 mm, 11.5 mm, 10.25 mm and 14.75 mm (at a concentration of 5%) respectively. The examination of main chemical constituents of ethyl acetate exctract of symbiotic fungi showed that it contains phenolic, terpenoid and steroid compounds. From the study, it can be concluded that fungi assosiated with marine sponge Acanthostrongylophora ingens are capable in producing of bioactive compounds, so it has potential as a source of anticancer and antibacterial compounds that are useful mainly in the fields of pharmaceuticals and health.
Key words: Marine sponge, symbiotic fungi, Acanthostrongylophora ingens, Anticancer activity, antibacterial activity
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