Home|Journals|Articles by Year|Audio Abstracts
 

Original Article

AJVS. 2016; 51(1): 162-169


Molecular Identification of Staphylococcus Aureus in Imported Frozen and Locally Slaughtered Meat

Ebeed. A. Saleh, Reham G. Abd El-Mohsen, Madiha S. Ibrahim.




Abstract

This work was planned to detect and identify Staphylococcus aureus in imported frozen meat (Brazilian and Indian) as well as Balady (local slaughter) meat using routine bacteriological examination and PCR. A total of 100 samples from Brazalian meat, 75 samples from Indian meat and 50 samples from Balady meat were analyzed. Our results showed that the prevalence of Staphylococcus aureus in Brazilian, Indian and Balady meat was 27%, 26.67 % and 22 %, respectively. Antimicrobial resistances of the S. aureus isolates showed sensitivity to penicillin, rifompion, ampicillin and novobiocin, while resistant to oxacillin, sulphatrimethoprim, vancomycin and Cefotoxin. PCR for the detection of the mecA gene of methicillin resistance was positive with the tested oxacillin resistant S. aureus isolates. Further, multiple antibiotic resistances were detected in 98% (57/58) of the isolates. Higher MAR index was detected from S. aureus isolates from the three types of meat and different resistance phenotypes were detected. PCR for the detection of the virulence genes; Sea, Seb, Sec, Sed and See genes, was performed. Sea and Sed genes were negative, while Seb, Sec, and See genes were detected in 16.66%, 33.33% and 50% of the tested isolates, respectively. These results collectively indicate that Brazilian, Indian as well as balady meat can harbor S. aureus with high percentage of resistance to oxacillin and a wide range of multiple antimicrobial resistance.

Key words: Staphylococcus aureus, MRSA, Frozen meat, Balady meat






Full-text options


Share this Article


Online Article Submission
• ejmanager.com




ejPort - eJManager.com
Refer & Earn
JournalList
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.