Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Research

Effect of Mifepristone, Sericin and Taurine in Tris Extender on Oxidative Markers and Quality of Fresh and Frozen-Thawed Bovine Spermatozoa

Devangana Chaturvedi, Arjun J Dhami, Dinesh V Chaudhari, Mohsin M Pathan.

This study was carried out on semen of 3 Gir and 3 Murrah bulls. Semen ejaculates with >75 % initial motility (n=7 per • 21/breed; total 42) were split-diluted at 100 million sperm per ml using Tris-citric acid-fructose-egg yolk-glycerol (TFYG) extender without and with Mifepristone (10 g/ml), Sericin (5 mg/ml) and Taurine (4 mg/ml) as additives. French mini straws were filled and sealed using IS4 system, and frozen in liquid nitrogen vapour using a programmable biofreezer after 4 hrs of equilibration. The straws were thawed in water bath at 37C for 30 sec. The overall mean per cent sperm motility observed in freshly diluted and frozen-thawed semen, irrespective of antioxidants, were 83.570.51 and 46.730.67 in Gir, and 83.150.54 and 48.810.82 in Murrah bulls, respectively. The corresponding per cent capacitated sperm (B+AR pattern) in chlortetracycline (CTC) fluorescence assay were 17.770.53 and 34.440.95 in Gir, and 18.000.51 and 31.200.82 in Murrah bulls. The mean seminal plasma MDA (mol/ml) activity on dilution and at post-thaw stage were 18.830.25 and 20.590.28 for Gir, and 19.320.26 and 21.760.31 for Murrah bulls, respectively. The corresponding means for SOD (U/ml) were 2.940.05 and 2.450.03 for Gir, and 2.350.06 and 1.970.05 for Murrah bulls, and the GPx (nmol/min/ml) 493.858.31 and 438.87 8.13 for Gir, and 508.828.71 and 452.838.20 for Murrah bulls, respectively. All the parameters differed significantly between stages and between extender-additives. MDA activity and capacitated sperm were significantly (p

Key words: Antioxidant Sericin, Mifepristone, Taurine, Bovine semen, Cryopreservation, Oxidative markers, Sperm motility, Cryocapacitation

Similar Articles

Study on the antioxidative mechanism of tocopherol loaded ethyl cellulose particles in thermal-oxidized soybean oil.
Liu Y, Ma X, Li J, Fan L, Huang S
Carbohydrate polymers. 2022; 276(): 118734

Copper ferrite nanoparticles induced cytotoxicity and oxidative stress in Channel catfish ovary cells.
Srikanth K, Nutalapati V
Chemosphere. 2022; 287(Pt 2): 132166

Striatal oxidative damages and neuroinflammation correlate with progression and survival of Lewy body and Alzheimer diseases.
Li H, Knight WC, Xu J
Neural regeneration research. 2022; 17(4): 867-874

Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cells.
King-Smith C
Methods in molecular biology (Clifton, N.J.). 2022; 2364(): 319-326

Antioxidant activity, stability, in vitro digestion and cytotoxicity of two dietary polyphenols co-loaded by β-lactoglobulin.
Ren Y, Liu H, Wang D, Liu T, Zhang R, Wu Y, Zhang Y, Han J, Liu M
Food chemistry. 2022; 371(): 131385

Full-text options

Latest Statistics about COVID-19
• pubstat.org

Add your Article(s) to Indexes
• citeindex.org

Covid-19 Trends and Statistics
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.

ScopeMed Web Sites