Abstract

The synergistic modification of the basic formulation for delivering natural active compounds aims to enhance stability, prevent degradation, and potentially influence their interaction with skin cells. Nanoliposomes are an effective delivery system, capable of preserving the integrity of the compounds and supporting targeted skin distribution. The system’s effectiveness will be evaluated for its antioxidant and anti-acne properties using the ethyl acetate fraction of Sonneratia caseolaris L. leaf extract. Liposomes were prepared by thin-layer hydration and then reduced and uniformed in size by ultrasonic and mini extruder into nanoliposome preparations. The preparation was evaluated descriptively and statistically based on all physical characteristics and stability tests of NANO-SERF-SCs preparation. The NANO-SERF-SCs produced were categorized as a small unilamellar vesicle with a particle size of 199.7 nm ± 3.61 with an even particle size distribution and spherical shape. The encapsulation efficiency obtained was 76.74% ± 2.80%. NANO-SERF-SCs were also stable under freeze-thaw cycle conditions, 25 times dilution, and 30 days of storage, with acceptable physical characteristics. The results of antioxidant testing using DPPH and ABTS methods show that NANO-SERF-SCs belong to the class of strong antioxidants with IC50 values close to the IC50 value of each positive control used. The results of the antimicrobial test using disc-diffusion showed that the NANO-SERF-SCs have anti-acne effectiveness that falls into the strong category because it has inhibition in the 10–20 mm range. The ethyl acetate fraction of S. caseolaris L. leaf extract can be formulated into a nanoliposome preparation and has good antioxidant and anti-acne activities.

Key words: Sonneratia caseolaris L., nanoliposomes, thin layer hydration, antioxidant, antiacne.