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Original Article

J App Pharm Sci. 2016; 6(12): 103-109


Phytochemical investigation of Phoenix canariensis Hort. ex Chabaud leaves and pollen grains and spathe volatile analysis.

Mohamed S. Hifnawy, Amr M. K. Mahrous, Rehab M. S. Ashour.




Abstract

Phoenix canariensis is a commonly grown, yet understudied, palm plant. The phytochemical screening of leaves and pollens revealed the presence of flavonoids, saponins, tannins, sterols and/or triterpenes. Quantitative estimation of constituents, revealed that the total polyphenolics were higher in the leaves (69.9) than in pollens (29.98) expressed in mg gallic acid equivalent/g d.wt, the total flavonoids calculated as rutin equivalent were (23.86 mg/g) in leaves and (17.20 mg/g) in pollens, the total tannins content was 55.18 and 3.31 mg tannic acid equivalent/g fresh wt, while the total steroids content were 2.6 and 12.4 mg β-sitosterol equivalent/g d.wt, in leaves and pollens, respectively. Eighteen phenolic compounds and ten flavonoids were identified by HPLC. GLC analysis of lipids, revealed identification of phytosterols (4.93 and 28.90%), saturated (35.35 and 40.56%) and unsaturated (62.42 and 59.01%) fatty acids in leaves and pollens, respectively. Proximate analysis revealed a total moisture content of (6.4 and 7.7 %), crude fiber (32.22 and 39.50%), total ash (12.1 and 8.1%) and acid insoluble ash (4.7 and 7.6 %) for leaves and pollens, respectively. Moreover, spathe headspace volatile analysis combined with GC-MS revealed the presence of fifty-two compounds constituting 72.84% of the total oil composition where α- copaene predominates (18.72%).

Key words: Phoenix canariensis, Lipids, Headspace volatiles, Flavonoids, Phenolics, Steroids.






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