Abstract

Sample pretreatment methods are a vital component in the pharmaceutical assays of analytes with the objectives of minimizing matrix effects and better recovery. The associated challenges involved are multistep procedures, time-consuming, labor-intensive, and a lack of selectivity. These limitations can be overcome by the advancement in novel extraction methods. These methods are preferred over traditional methods due to the ease of the clean-up procedure and the possibility of automation, miniaturization, and good recovery of target analytes. Therefore, the present review highlights the current progress in novel pretreatment methods and their applicability in the extraction of analytes from biological fluids. Various case studies have been covered about the pharmaceuticals and were summarized with critical parameters associated with method developments. In addition, considerable progress in novel methods for the extraction of biomarkers and diagnostics has been made. The integration of artificial intelligence and the adoption of green sampling approaches allow faster and reliable bioanalysis workflows. These developments have a significant impact in making bioanalysis more accessible, eco-conscious, and high accuracy in bioanalytical method developments.

Key words: Biomarkers, bioanalysis, biofluids, miniaturization, novel pretreatment methods, recovery