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In-vitro Study of MIRB Labeled Ovine Bone Marrow Derived Mesenchymal Stem Cells by MRI Technique

Gnanadevi R., Geetha Ramesh, Kannan T. A., Justin William B., Sathyan G., Sabiha Hayath Basha.

Abstract
Internalized MIRB was observed only after 72 hrs of incubation of the cells. The MIRB labelled cells showed decreased intensity when subcultured. Prussian blue staining was done to demonstrate the iron uptake of the cells. MIRB labelled cells showed positive reaction whereas, the unlabelled cells did not show any positive reaction. There was no significant difference in the viability of MIRB labelled BM-MSCs and ADMSCs compared to the unlabelled BM-MSCs and ADMSCs, respectively. The signal intensity in MIRB-labelled cells at passage 4 decreased with increasing concentrations of SPIOs. It is concluded that SPIONs could be used to label BM-MSCs at various passages and they emit signals sufficient to pickup at MRI T2 weighted images.

Key words: Labeling, Bone Marrow Derived Mesenchymal Stem Cells, Ovine, MRI Imaging



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