Home|Journals|Articles by Year|Audio Abstracts

Original Research

Int J One Health. 2016; 2(2): -

Emergence of extended spectrum beta-lactamases-producing strains belonging to CTX-M-1 class from intensive care units patients and environmental surfaces in Pakistan

Authors Name, Email and Affiliations Aqsa Ashraf Bukhari1 ‡, Muhammad Imran Arshad1 ‡, Shahbaz Raza1, Mariya Azam1, Sajjad-ur-Rahman1, Mashkoor Mohsin1* 1 Institute of Microbiology, University of Agriculture, Faisalabad, 38040, Pakistan ‡ Shared first authors on this work *Corresponding author: Dr. Mashkoor Mohsin, Institute of Microbiology, University of Agriculture, Faisalabad,-38000, Pakistan. E-mail: mashkoormohsin@uaf.edu.pk, Phone +92-30099659046 AAB email; Bukhari aqsa@yahoo.com MIA email; drimranarshad@yahoo.com SR email; shahbaz.invincible3182@gmail.com MA email; Mazam.87@gmail.com SUR email; sajjadur@uaf.edu.pk MM email; mashkoormohsin@uaf.edu.pk Authors Contributions AAB Sample collection and bacterial cultivation MIA Sample collection and biochemical confirmations SR PCR and gel electrophoresis MA DNA extraction and Quantification SUR Concept, article writing and revision MM Study Design, Writing and optimization of standards.


Aim: The emergence of multidrug resistant (MDR) bacteria is the most dangerous threat for the treatment of infectious diseases. The aim of this study was to detect and characterize extended spectrum beta lactamases (ESBL) and carbapenemase producing Klebsiella pneumoniae (K. pneumoniae) and Escherichia coli (E. coli) among patients and environment of Intensive Care Units (ICU) of three tertiary care hospitals in Pakistan.
Materials and Methods: A total of 82 samples from ICU’s patients and inanimate environment (injection trays, wash basins, door handles, hand swabs of professionals, ICU fridges) were screened for ESBL by culturing on CHROMagar-ESBL. ESBL and carbapenemases production were confirmed by double disc synergy test and Modified Hodge’s test, respectively. PCR was used to detect ESBL encoding genes blaCTX-M, blaCTX-M-1, blaCTX-M-2, blaCTX-M-9, blaTEM, blaSHV and carbapenemase genes blaKPC, blaNDM-1, blaOXA-48 and blaVIM.
Results: Overall ESBL production was found high 30/82 (36.5%) among isolates of which 15.8% K. pneumoniae and 20.7% E. coli were identified. All the K. pneumoniae and majority of E. coli isolates were MDR i.e. resistance to three or more antimicrobial categories. Molecular characterization showed the blaCTX-M-1 as the predominant genotype found in 17/21 (80%) of the isolates. None of the strains was found positive for carbapenemase encoding genes.
Conclusion: In conclusion, this study demonstrates the emergence of multidrug resistant ESBL producing strains among ICU patients and hospital environment, posing a serious threat for the control of nosocomial infections.

Key words: nosocomial infections, E. coli, K. pneumoniae, antimicrobial resistance, ESBL

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Refer & Earn
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.