Cryopreservation causes breach in the plasma membrane which culminates into loss of membrane permeability, change in membrane fluidity, exudation of acrosomal enzymes, and modification in mitochondrial membrane potential. Keeping these views in mind the current study was conducted to evaluate the apoptosis like changes or cryoinjuries in spermatozoa during cryopreservation. In the present study we collected semen from 4 Hariana bulls and after dilution semen was evaluated for various parameters, thereafter semen was cryopreserved. Various sperm characteristics were further evaluated 24 hrs after cryopreservation. The findings of the current study revealed that there is significant reduction in progressive motility, viability, acrosomal integrity, plasma membrane integrity and mitochondrial membrane potential of spermatozoa after semen cryopreservation. Thus we concluded that cryopreservation induced cryoinjuries in spermatozoa and investigation regarding mechanism involved in these changes is key factor to improve the success of semen cryopreservation.
Cryoinjuries, Cryopreservation, Hariana Bull, Semen