Dietary incorporation of whey proteins and galactooligosaccharides exhibits improvement in glucose homeostasis and insulin resistance in high fat diet fed mice -

J Complement Med Res. 2017; 6(3): 326-332

doi: 10.5455/jice.20170526091235

Praveen Kumar Kavadi, Ramesh Pothuraju, Raj Kumar Sharma, Jayasimha Chagalamarri, Gaurav Bhakri, Aswani Mallepogu.

Abstract
Background: The present study was planned to investigate the effectiveness of whey protein isolate (WPI) of high purity and a galactooligosaccharides (GOS) preparation on glucose homeostasis and insulin resistance under high fat diet (45.47% energy from fat) fed conditions in C57BL/6 mice. The mRNA expression of genes related to gluconeogenesis was also examined. Methods: Fasting blood glucose level, serum insulin & GLP-1 (ELISA) were measured; HOMA-IR determined in different treatment groups. mRNA expression of gluconeogenesis genes in liver and small intestine tissues analysed by qRT-PCR. Results: Dietary incorporation of WPI/GOS alone or in combination was observed to significantly resist (p Key words: High fat diet, glucose homeostasis, insulin resistance, whey protein isolate, galactooligosaccharides.