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Profiling of 6 mini STR loci (D1S1677,D2S441,D4S2364, D10S1248, D14S1434, D22S1045) on blood spots waited on various surfaces [Cesitli yuzeyler uzerindeki kan lekelerinde 6 mini str lokusunun (D1S1677, D2S441, D4S2364, D10S1248, D14S1434, D22S1045) profillendirilmesi]

Tugba Unsal, Gonul Filoglu, Havva Altuncul, Kadir Dastan, Ozlem Bulbul.

Abstract
Conventional STR loci are insufficient for successful analysis of DNA specimens from mass disasters or forensic evidence. In order to solve this problem, The National Institute of Standards and Technology (NIST) developed 26 new STR loci. The PCR products of these loci are less than 150 bp in size. Especially the PCR products of 6 loci (D1S1677,D2S441,D4S2364,D10S1248,D14S1434,D22S1045) are less than 125 bp in size. In this study, blood was dripped on various surfaces that may be found in crime scenes (jean, t-shirt, couch upholstery, towel, knife hilt, laminate parquet, door lock iron and grouting between tiles) and they were kept waiting for 1 week, 1 month, 3 months and 6 months. For the mentioned loci, the PCR conditions previously determined in the optimization and validation process were applied. At the end of this study, it was determined that the minimum DNA quantity of 6 mini SRT loci can be 0.1 ng/μl for identification of blood spots.

Key words: Forensic sciences, DNA analysis, waited biological samples, blood spots, miniSTR, D1S1677, D2S441, D4S2364, D10S1248, D14S1434, D22S1045



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