OBJECTIVE: To analyze relationship of vitamin D status with Age, Gender, Occupation, Serum
Calcium, ESR and Hemoglobin level in local population at Liaquat University of Medical &
STUDY DESIGN: Cross-sectional observational study.
DURATION OF STUDY: Liaquat University Hospital in Medical ward and Out Patient Department
(OPD) from 1st May 2015 - 1st December 2015.
METHODOLOGY: This cross-sectional observational study was conducted at Department of
Medicine, LUMHS Hyderabad. All cases were collected from OPD of medical ward and emergency,
after taking informed consent. A specifically designed semi structured proforma was
used to record demographic, history, examination and laboratory reports including serum calcium,
ESR, Hemoglobin and vitamin D level. Patients having chronic liver disease, ischemic
heart disease, cerebrovascular disease, road traffic accident and others critical illness were excluded.
Data entered into SPSS 20 version for analysis and p value.
RESULTS: A total of 160 patients were included in this study. The mean age of enrolled
participants was 40.2±13.7 years. Ninety seven (60.6%) were females with female to male ratio of
1.5:1. One hundred thirty (81.3%) patients were seen in out-patient department of institute. One
hundred and twelve (70%) patients are working at home. The mean hemoglobin level was 11±1.3
gm/dl, mean ESR 26.2±15.8, mean serum calcium 9.6±2 mg/dl and mean vitamin D3 level was
32±6.6 IU. Comparison of demographic and lab features of vitamin D deficient patients and with
normal vitamin D was summarized in Table I.
CONCLUSION: In Pakistan vitamin D deficient sample are more prevalent in all age group.
Study shows relationship of age, sex, occupation with serum hemoglobin level, erythrocyte
sedimentation rate and serum calcium level. Study results show that vitamin D deficient is more
in house workers, laborers, students and executive professional workers. Hemoglobin level
was found low in vit D deficient patients as compared to vit D normal population but serum
calcium, ESR were normal in both group of vitamin D deficient and controlled group of
Vitamin D, hemoglobin, age, gender, occupation