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Original Article

J App Pharm Sci. 2016; 6(12): 067-074


Modulatory Effect of Wheat Germ Oil on Intestinal Oxidative Stress and DNA damage Induced by Carbon tetrachloride in Mice

Hanan Saleh.

Abstract
Background/Aim: The liver is continuously linked to the gut via the portal vein supply. Gastrointestinal complications are directly associated with liver cirrhosis. Carbon tetrachloride (CCl4) caused liver toxicity is well documented in animal models, the very fewer search has been carried out on the intestinal damage in case of acute liver injury. Thus, this study aimed to investigate the intestinal alteration and subsequently the potential therapeutic role of Wheat Germ Oil (WGO) in the liver and small intestine after acute administration of CCl4. Material and Methods: Mice were randomly divided into 4 groups, control group, WGO group: received corn oil orally for 2 days then WGO (1400mg/kg) orally for 8 days, CCl4 group: received CCl4 orally for 2 days then corn oil for 8 days, CCl4 + WGO group: received CCl4 for 2 days then WGO for 8 days. Serum lipid profile, serum lactate dehydrogenase (LDH), intestinal oxidative stress enzymes, histopathological and DNA fragmentation assays were estimated. Results: Acute dose (50%, 1 mL/kg body weight) of CCl4-induced hyperlipidemia, hypocholesteremia, elevation in LDH, malondialdehyde (MDA), nitric oxide (NO) and intestinal DNA damage in addition to the reduction in the intestinal oxidative stress markers and an alteration in the mucosal architecture. On the contrary, WGO administration has the potency to protect not only the liver but also the small intestine in acute CCl4-induced tissue damage. The valuable effect is chiefly attributed to its mechanism of reducing the lipid profile and suppressing the oxidative stress that caused DNA damage Conclusion: WGO administration could markedly improve the liver and the small intestine from the CCl4 damage and consequently may be used as a therapeutic agent against the hepatic and intestinal toxicity.

Key words: Keywords: CCl4; wheat germ oil; small intestine; lipid profile; DNA damage



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