The aim of this study was to evaluate the antioxidant activity of hexane, ethyl acetate and methanol extracts of Aristolochia bracteolata Linn. Methanol extract showed increased scavenging activity on 2,2-diphenyl-picrylhydrazyl (DPPH) (IC50 270.12 ±1.12 µg/ml), hydroxyl (IC50 450.04 ± 0.71 µg/ml), nitric oxide (IC50 640.06 ± 1.24 µg/ml) and hydrogen peroxide (IC50 300.51 ± 0.67 µg/ml) radicals, as well as high reducing power. Methanol extract also showed strong suppressive effect on lipid peroxidation (IC50 400.01±1.23) and metal chelating ability of methanol extract showed moderate effect (IC50 680.09±0.33) when compared to the reference standard. The results obtained in the present study clearly indicate that methanol extract has a significant potential to use it as an antioxidant agent. In the present study the methanol extract ofAristolochia bracteolata has been subjected to GC-MS (Gas Chromatography-Mass Spectrometery) analysis. Sixty chemical constituents have been identified, The major peaks fromGC-MS results revealed the presence of seven different phytochemical constituents likedodecane (7.50%), tetradecane (7.31%), tridecane (6.59%), undecane (6.29%), pentadecane (3.99%), hexadecane (2.69%) and decane (1.49%) whereas some other minor peaks corresponds to the presence of other phytoconstituents.
Key words: Antioxidant, Aristolochia bracteolata, GC-MS, Phytoconstituents
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