Home|Journals|Articles by Year|Audio Abstracts

Original Article

High-density culture of Vero cells in novel perfusion bioreactor system

Ravindra Patel,Debjani Dasgupta,Madhavi Vernekar.

Cited by 0 Articles

Improvements in upstream bioprocessing single-use systems have allowed implementation of high-density cultures in standard workflows. This study shows the capability of the novel designed single-use bioreactors (SUBs) to accommodates very high surface area per unit volume and enable the cell culture scientists to achieve and maintain a stable, high-density perfusion culture of Vero cells, without compromising mixing characteristics and nutritional homogeneity. Conventionally, adherent cell lines have been cultivated in non-controlled, labor-intensive and space-demanding 2D surface technologies i.e.: roller bottles (RB) and multi-layer flasks. Fixed-bed and Packed-bed bioreactors are popular alternatives to culture high density adherent cell lines for vaccines and other biologics production. However, the limitations that restricts the use of fixed-beds are non-homogeneous cell seeding, huge mass transfer gradients, inability of maintaining nutritional homogeneity, limitation of bed size expansion and difficulties during scale-up of etc. In this study, we have shown the design and development of novel disc-based bioreactors in single-use format for high-density Vero cell culture. During the process run of 12 days, we have observed 4.97 population doublings and final cell density achieved was 0.438 million cells/ sq.cm from the seeding density of 0.014 million cells/ sq.cm. Vero cell growth observed in novel bioreactor was significantly higher than the traditional culture devices like roller bottles and multilayer flasks.

Key words: Single-use bioreactors (SUBs), Vero cell culture, Perfusion bioprocessing, 3D cell carriers, Non-woven polyester microfibers, Fixed-bed and packed-bed bioreactors, Programmable logic controller (PLC)

Full-text options

Share this Article

Online Article Submission
• ejmanager.com

ejPort - eJManager.com
Refer & Earn
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.