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Evaluation of chronic chlorpyrifos-induced reproductive toxicity in male Wistar rat: protective effects of vitamin C

Muftau Shittu, Suleiman F. Ambali, Joseph O. Ayo, Mohammed Y. Fatihu, Mohammed M. Sulaiman, Lukuman S. Yaqub.

Abstract
The aim of the present study was to evaluate the effect of vitamin C on reproductive toxicity, induced by chronic chlorpyrifos (CPF) exposure in male Wistar rats. Twenty adult male Wistar rats were divided into 4 groups of 5 animals in each group. Group I received soya oil (2 ml/kg); group II was given vitamin C only (100 mg/kg); group III was administered CPF only (10.6 mg/kg; ~1/8th LD50), while group IV was pretreated with vitamin C and then exposed to CPF, 30 min later. The regimens were administered by gavage once daily for 15 weeks. At the end of the treatment period, the animals were sacrificed by jugular venesection after light chloroform anesthesia, and sera obtained from the blood samples were analyzed for follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone concentrations. Pituitary gland and the testicular tissues of each rat were quickly dissected, removed and assayed for the levels of glycogen and acetylcholinesterase (AChE) activity. The right caudal epididymis was evaluated for spermatozoa concentrations. The results showed that decrease in concentrations of spermatozoa, luteinizing and follicle-stimulating hormones, testosterone, testicular glycogen, and inhibition of pituitary gland and testicular AChE activities caused by CPF were ameliorated by vitamin C.

Key words: Acetylcholinesterase; Chlorpyrifos; Reproductive hormones; Sperm count; Testicular histology; Vitamin C



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