Present of this study was designed to improve the antioxidant activity of milk thistle (salymarin) against inoculated mice with virulent strain of Corynebacterium Pseudotuberculosis by measuring the serum levels of Free Radicals Peroxinitrite and Malondialdhyde. Fifty mice were divided into five groups equally G1:10 mice served as control negative. G2 served as control positive challenged virulent C. pseudotuberculosis (5×109) CFU/ ml I/P. G3 giving plant extract of salybum marianam 350 mg / kg orally. G4 giving saly marin for thirty days and challenged with Corynebacterium pseudotuberculosis. G5 challenged with virulent C. pseudotuberculosis (5×109) CFU/ ml I/P after thirty days treated with plant extract for thirty days. At thirty days of experiment (5) mice were sacrefecied from each group and serum blood were collected for measuring oxidant levels (Peroxinitrite and Malondialdhyde ), also at sixty days of experiment all animal were sacrificed to determine the free radicals level (Peroxinitrite and Malondialdhyde). The results revealed that the serum levels of free radicals in group treated with milk thistle lower than serum levels treated with Corynebacterium psedotuberculosis. This study concludes that the antioxidant activity of milk thistle in prevent and treatment the oxidative stress.
Milk thistle, Corynebacterium psedotuberculosis and oxidative stress.
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