Abstract
This study was conducted to detect the prevalence of metallo-beta-lactamases (MBLs) and extended-spectrum beta-lactamases (ESBLs) in bacterial isolates from clinical and community settings in Delta State, Nigeria. Eighty four isolates obtained from blood, urine wound and stool of patients and community subjects were analyzed and identified by standard microbiological methods. Carbapenemase detection was carried out using modified Hodge test. EDTA-disc synergy test was used to detect MBL production. ESBLs production was determined by double disc synergy test (DDST). ESBL production was detected in 36.7% isolates of Escherichia coli, 37.5% of Pseudomonas aeruginosaand 75% of Klebsiellapneumoniae. Co-production of MBL and ESBL was observed in 31.82% of the isolates. The study observed 2 major troubling findings. The first is the prevalence and co-production of MBLs and ESBLs both in hospitalized patients and in isolates of healthy community children. Second, antibiotic resistance is no longer only associated with selective pressure but may stably persist in bacteria. There is therefore the need for antibiotic alternative research.
Key words: Keywords: Antibiotic resistance, Multidrug resistance, Healthy children, Beta-lactamases, Plasmids
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