Objective: We aimed to establish a high performance liquid chromatography (HPLC) method to measure plasma ADMA levels easily, sensitively and reliably.
Methods: We used fluorescence detection, 50 mM sodium acetate buffer/ methanol/ Tetrahydrofuran as a mobile phase (A, 82:17:1; B, 22:77:1, % v, respectively), gradient method, 338 and 425 nm excitation and emission wavelengths, respectively. Samples were derivatizated with o-Phthaldialdehyde.
Results: The recovery was found as 96 to 98,3 %. Intra-assay and inter-assay coefficients of variation (CV) were 2.3% and 2.7%, respectively.
Conclusion: These findings suggest that our method is suitable for routine determination of ADMA with more reliable.
Key words: ADMA, HPLC, Measurement
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