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Original Article

J Pak Med Assoc. 2016; 66(6): 658-661

Evaluation of phenotypic tests for detection of Amp C beta-lactamases in clinical isolates from a tertiary care hospital of Rawalpindi, Pakistan

Nadia Saad, Tehmina Munir, Maliha Ansari, Mehreen Gilani, Mahwish Latif, Amira Haroon.

Objective: To evaluate sensitivity and specificity of disc approximation test compared to three-dimensional extract test as a phenotypic gold standard test for detection of AmpC beta-lactamase producing Escherichia coli and Klebsiella pneumoniae.

Method: The cross-sectional validation study was conducted from November 2014 to April 2015 at Army Medical College, Rawalpindi, Pakistan. Extended spectrum beta lactamases (ESBLs) were isolated from various clinical specimens. Screening for AmpC beta-lactamases was done by using cefoxitin disc (30µg) showing inhibition zone diameter of

Key words: AmpC beta lactamases, Extended-spectrum-b-lactamase (ESBLs), Gram negative rods (GNRs)

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