Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Original Article

J App Pharm Sci. 2016; 6(12): 162-169


The impact of oxidative stress on Par2, Ptgs2 genes expression in rat duodenal epithelial cells under conditions of prolonged gastric hypochlorhydria and with administration of multiprobiotic

Alevtina Dranitsina, Kateryna Dvorshchenko, Dmytro Grebinyk, Lyudmila Ostapchenko.

Abstract
Development of dysbiosis is a key consequence of long hypoacidity, in which there is colonization of the gastrointestinal tract by pathogenic microflora that forms source of sustainable endogenous infection and leads to inflammation. The prevalence of free-radical processes of antioxidant defense system is an important pathogenetic link in the origin and development of various disorders. Because reactive oxygen species are important for transcription of Ptgs2 gene, the relationship between oxidative stress, antioxidant element and PAR2-mediated signaling pathways may take place under conditions of dysbiotic changes due to hypochlorhydria. To evaluate the intensity of free radical processes and their impact on Par2, Ptgs2 genes expression in rat duodenal epithelial cells under conditions of prolonged gastric hypochlorhydria and with administration of multiprobiotic. Four experimental groups were created with 8 rats in each. One group was a control, while others were injected with acid suppressant omeprazole or multi-strain probiotic preparation or with both compounds simultaneously for 28 days. Protein oxidative modification products, free SH-groups, metallothioneins content were measured in villus and crypt epithelial cells with standard biochemical assays. Level of Par2, Ptgs2 genes mRNA was determined with semi-quantitative RT-PCR. Prolonged inhibition of acid secretion in stomach was accompanied by significant elevation of free radical processes (intensification of protein oxidative modification, free SH-groups pool reduction, changes of metallothioneins concentration) and elevation of Par2 and Ptgs2 genes expression levels in rat duodenal epithelial cells. Reduction of free radical processes intensity; restoration of redox balance and the normalization of Par2, Ptgs2 gene expression pattern in the duodenum upon the simultaneous administration of the multiprobiotic Symbiter were observed. Par2 overexpression leads to activation Ptgs2 on the background of free radical processes over the antioxidant defense system in the development of inflammation in the duodenum through dysbiotic changes in the conditions of prolonged hypochlorhydria.

Key words: Hypochlorhydria; proton pump inhibitor; dysbiosis; multiprobiotic, gene expression



Similar Articles

Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases.
Vedam-Mai V
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 209-220

Hyperglycemia in acute ischemic stroke: physiopathological and therapeutic complexity.
Ferrari F, Moretti A, Villa RF
Neural regeneration research. 2022; 17(2): 292-299

Identification of key genes involved in axon regeneration and Wallerian degeneration by weighted gene co-expression network analysis.
Lu Y, Shan Q, Ling M, Ni XA, Mao SS, Yu B, Cao QQ
Neural regeneration research. 2022; 17(4): 911-919

Cerebral dopamine neurotrophic factor transfection in dopamine neurons using neurotensin-polyplex nanoparticles reverses 6-hydroxydopamine-induced nigrostriatal neurodegeneration.
Fernandez-Parrilla MA, Reyes-Corona D, Flores-Martinez YM, Nadella R, Bannon MJ, Escobedo L, Maldonado-Berny M, Santoyo-Salazar J, Soto-Rojas LO, Luna-Herrera C, Ayala-Davila J, Gonzalez-Barrios JA, Flores G, Gutierrez-Castillo ME, Espadas-Alvarez AJ, Martínez-Dávila IA, Nava P, Martinez-Fong D
Neural regeneration research. 2022; 17(4): 854-866

Baculoviral inhibitor of apoptosis protein repeat-containing protein 3 delays early Wallerian degeneration after sciatic nerve injury.
Cai M, Shao J, Yung B, Wang Y, Gao NN, Xu X, Zhang HH, Feng YM, Yao DB
Neural regeneration research. 2022; 17(4): 845-853


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites