The study was conducted for isolation and identification of various bacterial species from skin affection cases of freshwater fish of Punjab. Total 210 cases were examined during the period July 2009 to March 2010. Bacterial isolation was done from forty seven cases of skin affections. The various organs of fish showing gross lesions were collected aseptically. The isolation and identification of bacteria was done depending upon morphology, staining characteristics and biochemical testing using conventional methods as per the standard protocol. The bacterial species isolated were Aeromonas spp, Bacillus spp., Citrobacter spp., E. coli, Enterobacter spp., Flavobacter spp., Klebsiella spp., Lactobacillus spp., Micrococcus spp., Proteus spp., Pseudomonas spp., Salmonella spp., Staphylococcus spp., Streptococcus spp., Vibrio spp. and six gram negative bacterial isolates. Out of these Aeromonas was most predominantly isolated species followed by Vibrio. Gram negative bacteria were the most dominant species isolated from skin affection cases.
Key words: Aeromonas, Biochemical testing, Isolation and Vibrio.