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Original Article

J App Pharm Sci. 2017; 7(2): 147-155


Application of Hantzsch Reaction as a New Method for Spectrofluorimetric Determination of Some Cephalosporins

Mohamed A. El-Hamd, Ramadan Ali, Ahmed M. Haredy, Hytham M. Ahmed, Sayed M. Derayea, Osama H. Abdelmageed.

Abstract
A selective spectrofluorimetric method was described for determination of some cephalosporins namely, cefadroxil, cefaclor, cefixime, cefpodxime, and cefepime. These drugs were utilized as a source of amine group to react with acetylacetone and formaldehyde reagents via a Hantzsch reaction. The fluorescence intensity of the reaction product was measured at an emission wavelength of 482 nm after excitation at a wavelength of 415 nm. The linear calibration graphs were obtained in the concentration ranges of 1-100 g/mL for either cefadroxil or cefaclor and 1-50 g/mL for other three drugs. The limits of detection and quantitation were in the concentration range of 0.31-0.82 and 0.72-2.5 g/mL, respectively. The percent of relative standard deviation (at 50 g/mL concentration level, n = 5) were in the range of 0.41-1.70% and 1.51-1.95%, for intra-day and inter-day precision respectively. The method was successfully applied for the assay of some dosage forms of the cited drugs and the results obtained were comparable with those obtained by a reported method.

Key words: Spectrofluorimetric method. Cephalosporins. Acetylacetone. Formaldehyde. Hantzsch reaction. Dosage forms



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