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In vitro Xanthine Oxidase (XO) and Albumin Denaturation Inhibition Assay of Barringtonia racemosa L. and Total Phenolic Content Analysis for Potential Anti-Inflammatory Use in Gouty Arthritis

Nurul Izzati Osman, Norrizah Jaafar Sidik, Asmah Awal, Nurul Athirah Mohamad Adam, Nur Inani Rezali.

Abstract
Aim: The present study was conducted to evaluate the in vitro anti-inflammatory activities and total phenolic content (TPC) of methanolic extracts of infloresence axes, endosperms, leaves and pericarps of Barringtonia racemosa L.

Methods: The anti-inflammatory study was carried out by assessing the potential through xanthine oxidase (XO) and albumin denaturation inhibition assays. Meanwhile, the total phenolic content (TPC) in the extracts were assessed by Folin Ciocalteu assay.

Results: In the XO inhibition assay, the infloresence axes extract was found to exert the highest inhibition capacity at 0.1% (v/v) with 59.54% 0.001 inhibition followed by leaves (58.82% 0.001), pericarps (57.99% 0.003) and endosperms (57.20% 0.003) extracts. Similarly in the albumin denaturation inhibition assay, the infloresence axes extract had shown the greatest inhibition capacity with 70.58% 0.004 inhibition followed by endosperms (66.80% 0.024), leaves (65.29% 0.006) and pericarps extracts (43.33% 0.002). Meanwhile, for TPC analysis, leaves extract was found to have the highest phenolic content (53.94 0.000 mg GAE/g DW) followed by infloresence axes (31.54 0.001 mg GAE/g DW), endosperms (22.63 0.001 mg GAE/g DW) and the least was found in pericarps (15.54 0.001 mg GAE/g DW).

Conclusion: The results indeed verified the in vitro anti-inflammatory activities of B. racemosa and supported its potential to be used in alleviating gouty arthritis and XO-related diseases.

Key words: Barringtonia racemosa, anti-inflammatory activity, xanthine oxidase inhibition, albumin denaturation inhibition, total phenolic content



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