Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles

Open Access

Original Article

Dual effect of glucose on macrophage NADPH oxidase activity: a possible link between diabetes and tuberculosis

Dibyajyoti Banerjee, Priya Sharma.

Cited by (3)

Glucose is known to stimulate NADPH oxidase, an enzyme responsible for respiratory burst and intracellular killing of pathogens; but hyperglycemic state is associated with enhanced infections by invading microorganisms. In the present work we have examined the effect of glucose on macrophage NADPH oxidase activity. THP-1 cells and monocytes obtained from normal human subjects are incubated in different concentrations of glucose for a given period before and after conversion into macrophages by PMA treatment. Macrophage NADPH oxidase activity is measured and glycation of the membrane fraction is estimated in both the cases. The derived macrophages show enhanced glycation and significantly less enzyme activity when it is incubated in glucose for four days but the enzyme activity increases significantly when the macrophage precursor cells are incubated in high concentrations of glucose. This is supported by cell free experiment.

Key words: Diabetes mellitus; Glycation; Hyperglycemia; Macrophage; NADPH oxidase; Tuberculosis

Similar Articles

Boosting proteolytic pathways as a treatment against glycation-derived damage in the brain?
Taylor A, Bejarano E
Neural regeneration research. 2022; 17(2): 320-322

Hyperglycemia in acute ischemic stroke: physiopathological and therapeutic complexity.
Ferrari F, Moretti A, Villa RF
Neural regeneration research. 2022; 17(2): 292-299

Determination of Bioenergetic Parameters in Mycobacterium ulcerans.
Thomas SS, Pethe K
Methods in molecular biology (Clifton, N.J.). 2022; 2387(): 219-230

Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulcerans.
Thomas SS, Kalia NP, Pethe K
Methods in molecular biology (Clifton, N.J.). 2022; 2387(): 209-217

Use of Cocultures to Measure the Blood-Brain Barrier Permeability of Oxytocin.
Yamamoto Y, Harashima A, Munesue SI, Higashida H
Methods in molecular biology (Clifton, N.J.). 2022; 2384(): 247-255

Full-text options

Latest Statistics about COVID-19
• pubstat.org

Add your Article(s) to Indexes
• citeindex.org

Covid-19 Trends and Statistics
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.

ScopeMed Web Sites